| 摘要: |
| 以香蕉吸芽为实验材料，利用间歇浸没式植物生物反应器和传统组培方式对香蕉组培进行快繁研究的比较。以两种培养方式对香蕉苗进行培养，比较其生长形态、增殖倍数以及光合指标，采用单因素实验分析方法优化浸没频率，利用紫外分光光度法测定叶绿素含量。结果表明，浸没频率20 min/6 h是香蕉在植物生物反应器中快繁的最佳培养条件，并且两种培养方式下，香蕉组培苗的生长形态、增殖率、叶绿素含量以及光合性能有显著差异。利用间歇浸没式植物生物反应器系统可以提高香蕉组培苗增殖率，改善香蕉组培苗的生长状态；利用间歇浸没式植物生物反应器系统培育香蕉苗可以提高叶绿素含量以及光合性能。 |
| 关键词: 香蕉 植物组织培养 植物生物反应器 浸没频率 |
| DOI：10.14188/j.ajsh.20240821001 |
| 分类号:S668.1 |
| 基金项目:国家自然科学基金（82373981） |
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| Banana tissue culture and rapid propagation using intermittent immersion plant bioreactor |
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YAO Zhaoxu1, SHU Fuxing1,2, WANG Xinyao1, CHEN Xia1, CHEN Jishuang1,2
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1.Bioresource Institute for Healthy Utilization， Zunyi Medical University， Zunyi， Guizhou，563000， China;2.School of Biotechnology and Pharmaceutical Engineering， Nanjing Tech University， Nanjing 211816， Jiangsu， China
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| Abstract: |
| Banana sprouts were used as experimental materials to compare the rapid propagation of banana tissue culture using intermittent immersion plant bioreactors and traditional tissue culture method. Banana seedlings were cultured by the two cultivation methods， and then the growth morphology， multiplication ratio and photosynthetic index of banana seedlings were compared. The single-factor experimental analysis method was used to optimize immersion frequency， and chlorophyll content was measured by using UV spectrophotometry. The results showed that the immersion frequency of 20 min/6 h is the optimal culture condition for rapid propagation of bananas in the plant bioreactor. And the growth morphology， multiplication rate， chlorophyll content， and photosynthetic performance of banana tissue cultured seedlings are significantly different between the two cultivation methods. The use of the intermittent immersion plant bioreactors can significantly increase the multiplication rate of banana tissue cultured seedlings and improve their overall growth. Additionally， the cultivation using the intermittent immersion plant bioreactors enhances chlorophyll content as well as photosynthetic performance. |
| Key words: banana plant tissue culture plant bioreactor immersion frequency |
