| 摘要: |
丙谷二肽（L-alanyl-L-glutamine，Ala-Gln）是目前发现的一种在现代医疗健康领域极其重要的二肽，其进入人体后能够迅速水解成L-谷氨酰胺，促进蛋白质合成。生物合成丙谷二肽具有绿色、高效、安全的优点，主要的生物合成途径有微生物发酵法和生物催化法，其关键酶分别是L-氨基酸连接酶和α![]() ![]() -氨基酸酯酰基转移酶。通过敲除dpp、pep等基因来抑制二肽的降解，发酵法中Ala-Gln的最高水平为24.70 g/L，发酵时间为47 h。α![]() ![]() -氨基酸酯酰基转移酶以L-丙氨酸甲酯和L-谷氨酰胺为原料合成Ala-Gln，相关研究主要集中在酶的表达体系、定向进化、固定化等领域。α![]() ![]() -氨基酸酯酰基转移酶可以在25 min转化生成106.61 g/L的Ala-Gln，相较于微生物发酵法，该方法的反应时间更短，产物浓度更高，且无须ATP供能，是Ala-Gln工业化生产的首选工艺技术。 |
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| DOI：10.14188/j.ajsh.20240902001 |
| 分类号:TQ464 |
| 基金项目:浙江理工大学绍兴生物医药研究院开放基金（SXAB202007） |
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| Progress in biosynthesis and key enzymes of Ala-Gln |
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CHENG Kun1,2, WEI Hegeng3, YANG Weiqiang4, SHENG Qing1, YANG Zhongyi2
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1.College of Life Sciences and Medicine， Zhejiang Sci-Tech University， Hangzhou 310018， Zhejiang， China;2.School of Pharmaceutical Science， Taizhou University， Taizhou 318000， Zhejiang， China;3.Zhejiang Yongtai Technology Co.， Ltd.， Taizhou 317016， Zhejiang， China;4.Zhejiang Haizhou Pharmaceutical Co.， Ltd.， Taizhou 317016， Zhejiang， China
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| Abstract: |
L-alanyl-L-glutamine （Ala-Gln） is an important dipeptide in modern medical and health care. After entering the human body， it can be rapidly hydrolyzed into L-glutamine， which promotes protein synthesis. The synthesis of Ala-Gln dipeptide by biological method has the advantages of green， high efficiency and safety. The main biosynthetic pathways are microbial fermentation and biocatalysis， whose key enzymes are L-amino acid ligase and α![]() ![]() -amino acid ester acyltransferase. The degradation of dipeptide can be inhibited by knocking out genes such as dpp and pep， and the highest level of Ala-Gln in the fermentation method can reach 24.70 g/L after a fermentation time of 47 h. α![]() ![]() -amino acid ester acyltransferase synthesizes Ala-Gln from L-alanine methyl ester and L-glutamine， and the related research mainly focuses on the enzyme expression system， directional evolution， and immobilisation. α![]() ![]() -amino acid ester acyltransferase can convert and produce 106.61 g/L of Ala-Gln in 25 min. Compared with microbial fermentation， this method has a shorter reaction time， higher product concentration， and does not need ATP for energy supply， making it the preferred process technology for industrial production of Ala-Gln. |
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