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| 葡萄藤叶青贮中乳酸菌的分离与鉴定 |
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郭雪峰1,2,尹雪1,刘俊峰1,2,彭雨1,普宣宣1,张秀萍1,2,席琳乔1,2,张苏江2
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1.塔里木大学动物科学学院;2.新疆生产建设兵团塔里木畜牧科技重点实验室，新疆 阿拉尔843300
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| 摘要: |
| 为了筛选出性状优良的乳酸菌菌株，本试验以实验室纯培养方式从葡萄藤叶自然发酵的青贮中分离鉴定乳酸菌。经过菌落形态、细胞形态、生理生化鉴定和16S rDNA基因序列以及系统发育树的分析，从葡萄藤叶青贮中分离出5株乳酸菌，其中菌株P-1.2和P-2.2为戊糖乳杆菌（Lactobacillus pentosus），菌株P-1.7为戊糖片球菌（Pediococcus pentosaceus），菌株P-2.1为粪肠球菌（Enterococcus faecium），菌株P-2.3为短乳杆菌（Lactobacillus brevis）。测定产酸速率和生长速率，发现菌株P-2.1的产酸能力和生长性能最好。 |
| 关键词: 葡萄藤叶 青贮饲料 乳酸菌 分离 鉴定 |
| DOI：10.14188/j.ajsh.2019.01.011 |
| 分类号:S816.32 |
| 基金项目:新疆生产建设兵团科技创新中青年领军人才项目2016BC001;新疆生产建设兵团畜牧科技重点实验室项目HS201613新疆生产建设兵团科技创新中青年领军人才项目（2016BC001）;新疆生产建设兵团畜牧科技重点实验室项目（HS201613） |
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| Isolation and identification of lactic acid bacteria in the silage of grape vine leaves |
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GUO Xuefeng1,2,YIN Xue1,LIU Junfeng1,2,PENG Yu1,PU Xuanxuan1,ZHANG Xiuping1,2,XI Linqiao1,2,ZHANG Sujiang2
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1.College of Animal Science ＆Technology, Tarim University, Alar 843300, Xinjiang, China;2.Key Laboratory of Tarim Animal Husbandary Science and Technology of Xinjiang Production ＆Construction Crops, Alar 843300, Xinjiang, China
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| Abstract: |
| In order to screen out lactic acid bacteria with excellent characters, the laboratory pure culture method was used to isolate and identify the bacteria from the silage of grape vine leaves. Through the colony morphology, cell morphology, physiological and biochemical identification, 16S rDNA gene sequence analysis and phylogenetic tree analysis, five strains were isolated from the silage of grape vine leaves. Strain P-1.2 and strain P-2.2 were Lactobacillus pentose, strain P-1.7 was Pediococcus pentosaceus, strain P-2.1 was Enterococcus faecium and strain P-2.3 was Lactobacillus brevis. By measuring the rate of acid production and growth rate, it was found that strain P-2.1 had the best acid producing ability and growth performance. |
| Key words: grape vine leaf silage lactic acid bacterium isolation identification |
