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| ISSR标记技术在莲藕遗传研究中的运用 |
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汪岚1,3,韩延闯1,3,彭欲率1,3,滕彩珠1,3,周明全2,3,胡中立1,3,宋运淳1,3
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1. 武汉大学发育生物学教育部重点实验室,武汉,430072;2. 武汉大学莲藕中心,武汉,430072;3. 武汉大学发育生物学教育部重点实验室,武汉,430072;武汉大学莲藕中心,武汉,430072
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| 摘要: |
| 利用ISSR技术对12个莲藕品种进行了DNA多态性分析.从65个随机引物中筛选出7个引物扩增基因组DNA,共获得91条带,其中75条为多态性标记,每个引物平均提供13个标记信息.由UPMGA方法得到的聚类分析结果表明了12个品种间的亲缘关系,聚类结果与它们的系谱关系非常吻合. |
| 关键词: 莲藕 ISSR 多态性 亲缘关系 |
| DOI： |
| 分类号:Q943 |
| 基金项目:国家科技攻关项目，湖北省科技攻关项目，湖北省武汉市科技攻关项目 |
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| Application of ISSR Technique to Genetic Research of Lotus Root |
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| Abstract: |
| The ISSR analysis was conducted in 12 lotus. From 65 random primers, 7 primers were selected and then amplified the 12 genomic DNAs. 91 RAPD fragments were generated, 75 of them were polymorphic marks. Every primer offered 13 markers on average. The molecular phylogenetic tree conducted by UPMGA method and showed the genetic relationship among 12 lotus, which was better identical with pedigree analysis. |
| Key words: lotus Inter Simple Sequence Repeats polymorphism genetic relationship |
