| 摘要: |
| 为了探讨超低温反复冻融对药用植物种子活力的影响，选取了红花、玉米和黑豆3种药用植物的成熟种子作为实验对象，通过设置不同的冻融置留时间和次数进行实验，评估种子的生活力、发芽率以及生理生化指标的变化特性。结果显示，冻融置留时间和次数对种子的生活力和发芽率影响不显著，尤其是玉米种子，尽管发芽率有所下降，但仍保持在90%以上。此外，种子的过氧化物酶和过氧化氢酶活性在置留0 h至2 d内变化显著，之后趋于稳定，这可能是由于超低温冷冻胁迫初期影响了种子活性氧清除系统的能力。随着置留时间的延长，种子内部酶系统逐渐恢复平衡。研究还发现，冻融过程中丙二醛含量和电导率无显著变化，表明反复冻融未对药用植物种子造成严重损害。综合分析发现，液氮超低温反复冻融对正常性药用植物种子活力无显著影响，为药用植物种质资源的长期保存提供了理论和实践支持。 |
| 关键词: 超低温保存 反复冻融 药用植物种子 种子活力 |
| DOI：10.14188/j.ajsh.20240704001 |
| 分类号:Q4-33 |
| 基金项目:中国医学科学院医学与健康科技创新工程（2021-I2M-1-032） |
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| Impact of cryopreservation freeze-thaw cycles on the viability of medicinal plant seeds |
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HUANG Meiqin, ZENG Lin, FU Li, SUN Zheng, HE Liu
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Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine， Hainan Branch of the Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences and Peking Union Medical College， Haikou 570311， Hainan， China
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| Abstract: |
| In order to investigate the effects of cryopreservation freeze-thaw cycles on the viability of medicinal plant seeds， mature seeds of three medicinal plants， namely Carthamus tinctorius L.， Zea mays L. and Glycine max（L.）Merr.， were selected as experimental subjects. The viability， germination rate and physiological and biochemical indexes were evaluated by setting different freeze-thaw retention times and frequencies. The results showed that the effects of freeze-thaw retention time and frequency on seed viability and germination rate are not significant， especially for Zea mays L. seeds， which still remain above 90% despite a decrease in germination rate. In addition， the peroxidase and catalase activities of these seeds vary significantly from 0 h to 2 days， and then stabilize， which may be due to the initial impact of the ultra-low temperature freezing stress on the seed capacity of the reactive oxygen species scavenging system. With the prolongation of the retention time， the internal enzyme system of the seeds gradually regain its balance. It is also found that there are no significant changes in malondialdehyde content and conductivity during the freezing and thawing process， which indicate that cryopreservation freeze-thaw cycles do not cause serious damage to the seeds of medicinal plants. It is concluded that the freeze-thaw cycles of liquid nitrogen at ultra-low temperatures have no significant effect on the seed viability of normal medicinal plants， which provides theoretical and practical support for the long-term conservation of medicinal plant germplasm resources. |
| Key words: cryopreservation freeze-thaw cycles seeds of medicinal plants seed viability |
