| 摘要: |
| 从源自红树根际土壤的一种球腔菌属菌株Mycosphaerella sp.HS-47发酵产物中分离出具有抗菌活性的次生代谢产物。综合运用多种色谱方法，从乙酸乙酯萃取物中分离得到7个化合物，将其与波谱数据进行比对，这7个化合物分别被鉴定为ergost-7，9（11），22-trien-3β，5α，6α-triol（1）；ergost-7，22-diene-3β，5α，6α-triol（2）；6，9-epoxy-ergosta-7，22-dien-3-ol（3）；3β，5α，9α-trihydroxy‐（22E，24R）-ergosta-7，22-dien-6-one（4）；22E-7α-methoxy-5α，6α-epoxyergosta-8（14），22-dien-3β-ol（5）；5α，6α-epoxy-（22E，24R）-ergosta-8，22-dien-3β，7α-diol（6）和3β，5α-dihydroxy-10α-methyl-6β-acetoxy-ergosta-7，22-diene（7）。抗菌活性结果表明，化合物1~7具有抗菌活性，其中化合物5和化合物6活性最为显著，对耐甲氧西林金黄色葡萄球菌S.aureus ATCC43300的最小抑制浓度均为1.560 μg/mL。该研究首次从球腔菌属菌株HS-47中分离出的甾体类化合物，其具有不同程度的抗菌活性。 |
| 关键词: 球腔菌属菌株HS-47 麦角甾 分离与鉴定 抗菌活性 |
| DOI：10.14188/j.ajsh.20240531001 |
| 分类号:Q935 |
| 基金项目:国家自然科学基金（81903772） |
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| Isolation and identification of ergosterols with antimicrobial activity in the fermentation products of Mycosphaerella sp.HS-47 |
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PAN Chao, HUA Silu, LIU Ping, MIAO Li, JIANG Wei
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College of Environmental Science and Engineering， Yangzhou University， Yangzhou 225127， Jiangsu， China
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| Abstract: |
| The secondary metabolites with antimicrobial activity were isolated from the fermentation products of Mycosphaerella sp.HS-47 derived from mangrove rhizosphere soil. Seven compounds were obtained from the EtOAc extract by means of various chromatographic techniques. The structures of the seven compounds were identified as ergost-7，9（11），22-trien-3β，5α，6α-triol（1）； ergost-7，22-diene-3β，5α，6α-triol（2）； 6，9-epoxy-ergosta-7，22-dien-3-ol（3）； 3β，5α，9α-trihydroxy-（22E，24R）-ergosta-7，22-dien-6-one（4）； 22E-7α-methoxy-5α，6α-epoxyergosta-8（14），22-dien-3β-ol（5）； 5α，6α-epoxy-（22E，24R）-ergosta-8，22-dien-3β，7α-diol（6），and 3β，5α-dihydroxy-10α-methyl-6β-acetoxy-ergosta-7，22-diene（7）， respectively， by comparison with spectroscopic data in the literature. The results of antimicrobial activity showed that compounds 1~7 have antimicrobial activity. Compound 5 and compound 6 have the most significant activity， with a minimum inhibitory concentration of 1.560 μg/mL for methicillin-resistant S.aureus ATCC43300. The ergosterols isolated from Mycosphaerella sp.HS-47 are reported for the first time， and they have varying degrees of antimicrobial activity. |
| Key words: Mycosphaerella sp.HS-47 ergosterols isolation and identification antimicrobial activity |
