| 摘要: |
| 下丘脑和垂体调节母鸡的生殖生理和产蛋性能。为了解其产卵调控机制，利用RNA-seq技术对高产和低产庄河大骨鸡的下丘脑和垂体组织进行测序和分析，获得SNP/InDel位点，并进行数据统计及生物信息学分析，以鸡基因组作为参考，对SNP所在基因进行GO富集分析。结果显示：SNP/InDel纯合子变异体数量高于杂合子变异体数量，垂体组织中的SNP/InDel数量高于下丘脑组织；SNP类型转换种类少于颠换，但转换类型的数量却远远高于颠换；垂体组织中的SNP所在基因的GO富集程度与生产性能呈负相关关系（P<0.05），下丘脑组织中的SNP所在基因的GO富集程度与生产性能呈正相关关系（P<0.05）。研究结果为大骨鸡的选育以及改善其生产性能的研究提供了理论依据。 |
| 关键词: 大骨鸡 转录组测序技术 单核苷酸多态性 插入缺失标记 基因本体富集 |
| DOI：10.14188/j.ajsh.2021.02.008 |
| 分类号:S831.2 |
| 基金项目:国家自然科学基金（31601914；31301971）；辽宁省自然科学基金（2019-ZD-0798）；国家大学生创新创业训练计划项目（201910160012；201910160004） |
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| SNP/InDel analysis of pituitary and hypothalamus of Dagu chicken based on RNA-seq technology |
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ZHANG Jiehui, DAI Tianshu, ZOU Shuang, WANG Chunqiang, MA Wei
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College of Animal Husbandry and Veterinary Medicine， Jinzhou Medical University， Jinzhou 121001， Liaoning， China
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| Abstract: |
| The reproductive physiology and laying performance of laying hens are regulated by the hypothalamus and pituitary. To understand the mechanism of egg laying regulation， we sequenced and analysed the hypothalamus and pituitary tissues in high- and low-yielding laying Chinese Dagu chickens （CDC） using RNA-seq， and got the SNP/InDel sites， then conducted data statistics and bioinformatics analysis using the chicken genome as a reference to analyze the genes of SNP. The result showed that the number of SNP/InDel homozygous variants was higher than that of heterozygous variants， and the number of SNP/InDel in pituitary tissues was higher than that in hypothalamus tissues； the SNP conversion type was less than transversion， but the number was inverse； the GO enrichment degree of SNP gene in pituitary tissues was negatively correlated with its production performance（P<0.05）， but the GO enrichment degree of SNP gene in hypothalamus was positively correlated with the production performance（P<0.05）. The results provide a theoretical basis for breeding Dagu chickens， and improve their production performance. |
| Key words: Dagu chicken RNA sequencing（RNA-seq） single nucleotide polymorphism （SNP） insertion-deletion（InDel） gene ontology enrichment |
