| 摘要: |
| 在5L发酵罐上用正交试验检测不同温度、pH和接种量对魔芋葡甘聚糖酶发酵生产的影响。试验表明,产酶的最佳条件为温度50℃,pH5.5～6.0,接种量10%,发酵前期搅拌速度100r·min-1,通气量20L·h-1,6h后搅拌速度改为50r·min-1,通气量10L·h-1,在此条件下获得的酶比活力为4.812×106U·g-1,总活力达到7.810×106U·L-1。培养8h后细菌生物量、产物含量迅速提高,24h达到顶峰时期。发酵动力学属于偶联型。 |
| 关键词: 魔芋 葡甘聚糖酶 葡甘低聚糖 发酵 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Preliminary Studies on Conditions of Experimental Production of Konjac Glucomannanase |
|
|
| Abstract: |
| Based on rotating shaker culture, the optimum conditions of konjac glucomannanase on the 5L fermenter were selected through an orthogonal experiment. The results indicated the optimum conditions were temperature at 50℃, pH 5.5～6.0 and pitching rate of 10%.At the beginning, stirring velocity was 50 rpm and aeration rate was 20 L·h -1. After six hours, stirring velocity was 100 rpm and aeration rate was 10 L·h -1. Under above conditions, the enzyme specific activity was high up to 4812 U·mg -1, the total activity was 7810 U·mL -1. After 8 hours, the biomass and product yield rose up promptly and reached the summit after 24 hours. According to the data, the fermentation was of coupling kinetics. |
| Key words: Amorphophallus rivieri Durieu konjac glucomannanase konjac oligosaccharide fermentation |
